质谱法分析蛋白的基本原理是通过电离源将蛋白质分子转化为离子,然后利用质谱分析仪的电场、磁场将具有特定质量与电荷比值(M/Z)的蛋白质离子分离开来,经过离子检测器收集分离的离子,确定离子的M/Z值,分析鉴定未知蛋白。通常结合相应的处理及其他技术,能够比较准确、快速地鉴定蛋白质。
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