免疫共沉淀(Co-Immunoprecipitation)是以抗体和抗原之间的专一性作用为基础的用于研究蛋白质相互作用的经典方法。是确定两种蛋白质在完整细胞内生理性相互作用的有效方法。基本原理:在细胞裂解液中加入抗原特异性的抗体,抗体、抗原以及与抗原具有相互作用的蛋白质通过抗原与抗体之间的免疫反应形成免疫复合物,洗脱收集复合物,SDS-PAGE电泳,western blot或质谱可验证或鉴定与抗原互作的蛋白质。
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